Analysis of growth curves of indigenous male Venda and Naked Neck chickens

The objective of this work was to estimate and compare the growth curve parameters for live weight of indigenous Venda and Naked Neck chickens and carry out some analyses to test the existence of differences in the growth pattern between these breeds. The data were collected from the two breeds of chickens that were reared from day-old to 21 weeks of age. Two hundred chickens (100 of each breed) were used. Three different non-linear models, namely Gompertz, Logistic and Richards, were used to define the growth curves of the chickens. Models were compared using Coefficients of determination (R2 values), A parameter values, the Durbin-Watson Statistic (DW) test for autocorrelation, computing difficulty based on the number of iterations needed for convergence and size of residual variances. The R2 were high for all models: 0.996, 0.990 and 0.997 for Gompertz, Logistic and Richards, respectively. Residual variances were 2240, 5924 and 1154 for Gompertz, Logistic and Richards, respectively. The Gompertz model was observed to be suitable for explaining the growth of the chickens. Breed differences were observed in the growth parameters of chickens. The Venda breed was observed to be late maturing and heavier at maturity while the Naked Neck was shown to have a higher growth rate, reaching maturity earlier but attaining a lighter mature weight. Keywords: Growth parameters, growth models, breed differences South African Journal of Animal Science Vol. 37 (1) 2007: pp. 21-2

Effects of sex and stocking density on the performance of broiler chickens in a sub-tropical environment

The current study investigated the effects of sex and stocking density and their interaction on growth performance and carcass characteristics of broiler chickens in a semi-arid sub-tropical environment. A total of 1008 day-old Cobb Avian48 chicks, namely 462 males and 546 females, were reared at final stocking densities of 30, 35, and 40 kg bodyweight per square metre (BW/m2) corresponding to 44, 51 and 59, and 52, 61 and 69 chicks per pen for males and females, respectively, during a 42-day production period. The experiment was a 2 × 3 factorial completely randomized design, with each sex-stocking density combination being replicated three times. After the initial weighing, the birds were weighed individually [grams (g)] each week until the 42nd day. At day 42, four birds from each pen were randomly sampled and slaughtered, and carcass characteristics were measured after 48 hours, and expressed as percentages of bodyweight. Growth performance and carcass characteristic parameters were subjected to analysis of variance using the general linear model procedures of Minitab statistical software version 17. Treatment means were compared using Tukey’s test (at α = 0.05). Males gained more weight and were significantly heavier at slaughter age (2649 ± 43.1 g) than females (2270 ± 43.1 g). There was a progressive reduction in feed intake with increasing stocking density, but neither sex nor stocking density influenced feed conversion ratio and mortality rate. Sex significantly affected dressing percentage and carcass parts percentages, namely breast, neck, shank, heart and abdominal fat and thigh, liver and gizzard. On the other hand, stocking density significantly influenced dressing percentage and carcass parts percentages such as breast, thigh, drumstick, neck, shank, liver and gizzard. There was a significant sex x stocking density interaction effect on percentages of thigh and liver. It is therefore suggested that for efficient 42-day production cycles of broilers in the subtropics, Cobb Avian48 males reared at the stocking density of 40 kg BW/m2 could be considered the best option.Keywords: Carcass characteristics, Cobb Avian48, feed conversion ratio, mortalit

The calcimimetic R-568 induces apoptotic cell death in prostate cancer cells

<p>Abstract</p> <p>Background</p> <p>Increased serum level of parathyroid hormone (PTH) was found in metastatic prostate cancers. Calcimimetic R-568 was reported to reduce PTH expression, to suppress cell proliferation and to induce apoptosis in parathyroid cells. In this study, we investigated the effect of R-568 on cellular survival of prostate cancer cells.</p> <p>Methods</p> <p>Prostate cancer cell lines LNCaP and PC-3 were used in this study. Cellular survival was determined with MTT, trypan blue exclusion and fluorescent Live/Death assays. Western blot assay was utilized to assess apoptotic events induced by R-568 treatment. JC-1 staining was used to evaluate mitochondrial membrane potential.</p> <p>Results</p> <p>In cultured prostate cancer LNCaP and PC-3 cells, R-568 treatment significantly reduced cellular survival in a dose- and time-dependent manner. R-568-induced cell death was an apoptotic event, as evidenced by caspase-3 processing and PARP cleavage, as well as JC-1 color change in mitochondria. Knocking down calcium sensing receptor (CaSR) significantly reduced R-568-induced cytotoxicity. Enforced expression of Bcl-xL gene abolished R-568-induced cell death, while loss of Bcl-xL expression led to increased cell death in R-568-treated LNCaP cells,.</p> <p>Conclusion</p> <p>Taken together, our data demonstrated that calcimimetic R-568 triggers an intrinsic mitochondria-related apoptotic pathway, which is dependent on the CaSR and is modulated by Bcl-xL anti-apoptotic pathway.</p

Prodrug Strategy for PSMA-targeted Delivery of TGX-221 to Prostate Cancer Cells

TGX-221 is a potent, selective, and cell membrane permeable inhibitor of the PI3K p110β catalytic subunit. Recent studies showed that TGX-221 has anti-proliferative activity against PTEN-deficient tumor cell lines including prostate cancers. The objective of this study was to develop an encapsulation system for parenterally delivering TGX-221 to the target tissue through a prostate-specific membrane aptamer (PSMAa10) with little or no side effects. In this study, PEG-PCL micelles were formulated to encapsulate the drug, and a prodrug strategy was pursued to improve the stability of the carrier system. Fluorescence imaging studies demonstrated that the cellular uptake of both drug and nanoparticles were significantly improved by targeted micelles in a PSMA positive cell line. The area under the plasma concentration time curve of the micelle formulation in nude mice was 2.27-fold greater than the naked drug, and the drug clearance rate was 17.5-fold slower. These findings suggest a novel formulation approach for improving site-specific drug delivery of a molecular-targeted prostate cancer treatment